Keep an eye on small stacks that are liable to go all in on anything from a monster to ace small kicker, if you are going to call make sure you’ve got plenty of chips in the first place.
Like in casino poker, bigger stacks tend to get bigger respect, especially from new players. So always keeping a decent amount of chips "in front of you" is a good idea. However, you should always have enough chips available in your bank to jump into a different game. If you keep your whole bankroll in front of you, you can't buy-in to second game without cashing out of the first.
Playing Promptly
If you are a winning player, a smooth, prompt game is almost always in your favor. The more hands you are dealt in, the more money you make. So be sure to check the "muck losing hands" and "auto-post blinds" buttons. There are times you would want to uncheck the auto-post option (like if you go to answer the doorbell or the game gets short and you consider quitting) but I can't think of any reason to ever uncheck the muck losing hands button. Also, if you do leave your computer, check the "sit out next hand" button rather than getting dealt in and letting yourself "time out" when it comes to you to act. While it's rude to make people wait for you when you are not even in the room, you also shouldn't slow down a game in any case because a slower game is generally a less profitable game.
Perhaps 5% of online players are very rude. Sometimes literally half the game is spent repeatedly waiting for the same self-absorbed player to act on his hand. While this obnoxious behavior might tilt opponents a bit, creating enemies and being a jerk is seldom profitable in the long run. Playing promptly is the courteous and profitable thing to do.